|
KMID : 0603520020070010001
|
|
Journal of Korean Association of Cancer Prevention
2002 Volume.7 No. 1 p.1 ~ p.5
|
|
|
Effects of Green Tea-Drinking on CYPIAs in Different Tissues
|
|
Yang Mi-Hi
Lee In-Seon
Yoshikawa Masahiro
Arashidani Keiichi
Kawamoto Toshihiro
|
|
|
Abstract
|
|
|
To study chemopreventive mechanism of green tea (GT), we studied the effects of GT-drinking on hepatic, pulmonary, and renaI CYP1As against 3-methylcholand1rene(MC), which is a CYP1A-inducing arcinogen. Pre-and concurrent 2% GT drinking (1ldays) was conducted in arylhyrocarbon receptor (AR) responsive C57 BL/6 mice. As results, GT drinking reduced MC-induced pulmonary ethoxyresorufin-O- demethyIase (EROD)-activity, which is a probe for total CYPlAs-activity including CYPlAl and lA2. In contrast, GT drinking enhanced MC-induced hepatic EROD-activity-EROD activity in kidney was lower than those in lung or liver and GT drinking did not affect MC-induced renal EROD activity. These results suggest that the different effects of GT on CYPlAs are diverse in different tissues. Arylhydmcarbon hydroxyIase (AHH) activity, which is known to ref1ect CYPlAl activity, was not different according to GT drinking in lung, liver, or kidney.
Therefore, CYPlA2 or other microsomal enzymes rather than CYPlA1 might be affected by GT in lung, liver and kidney. However, the changes of EROD activity by GT were not confirmed with CYP1A antibody. Our results suggest that susceptibility to chemopreventive GT against CYPlA-biotfansformed carcinogens might be different in different tissues.
|
|
|
KEYWORD
|
|
|
Green tea, CYPlA, Chemoprevention
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|